If you are satisfied with the result, you can export your project to a GIF document or share it by email or through iMessage (in this case the GIF file will be automatically exported and sent to the respective applications). This way you can make the necessary adjustments on the spot. To check if everything is running smoothly, PicGIF comes with a built-in preview option. PicGIF also enables you to add captions to your slides, and you get to quickly customize the writing by changing the font style, size, alignment, outline or shadow. Moreover, you can adjust the frame delay and the slides sequence. To get started, adjust the canvas size, change the image's orientation (landscape or portrait), set up use canvas fill mode and the background color. However, note that PicGIF can work with up to 300 images at the same time. The best part is that the app allows you to use multiple file types in the same project.
The app is able to work with all sorts of image files, such as JPEG, TIFF, BMP, PNG, TGA, RAW, PIC and more.Īt the same time, PicGIF provides support for most popular video formats like MOV, MPEG, MP4, M4V, 3GP, AVI and more. PicGIF is able to import your own photos collections, or it can extract slides from a video file.
PICGIF LITE MAC
PicGIF is a Mac app designed to help you deal with the “technical” details. Structural insights into DNA cleavage activation of CRISPR-Cas9 system. Nature Communications, 8(1).GIF animations can be used to convey ideas in a compressed manner: for example, you can combine various photos in order to relate a vacation story in a a matter of seconds. : Huai, C., Li, G., Yao, R., Zhang, Y., Cao, M., Kong, L. Outer-membrane vesicles from Gram-negative bacteria: Biogenesis and functions. Nature Reviews Microbiology, 13(10), 605-619. : Jmol: an open-source Java viewer for chemical structures in 3D. The app says you can drag and drop onto the pop up screen on opening the app, but it. The picGIF app accepts many different image formats and all common video formats. Trying it out, I was able to make an animated GIF in less than five minutes start to finish, and you can too Importing. Crystal Structure of the Minimal Cas9 from Campylobacter jejuni Reveals the Molecular Diversity in the CRISPR-Cas9 Systems. It’s a job made much easier with the app PicGIF Lite. : Yamada, M., Watanabe, Y., Gootenberg, J. The data is calculated assuming cuboid shape of Cas9 proteins and perfect sphere shape of OMVs. As shown, CjCas9’s small size allows it to be carried in great amount during the transmission and is, therefore, more suitable to be used in OMV-mediated gene editing.įigure.2 Estimated quantity of Cas9s encapsulation by OMVs. The difference between the results is shown in Figure.2. The result shows an estimated bounding box volume of 1430 nanometers cube. In addition, as supplementary material for our choice in the type of Cas9s, we also estimated the number of SgCas9s one OMV can cargo through the same method. The approximate volume of CjCas9, approximately 700 nanometers cube(82x96x88), is determined by the bounding box volume obtained by Jmol. To provide a more statistical explanation of the Cas9 carrying ability of OMVs, we use a simulant model to calculate the amount of Cas9 proteins one OMV can carry. OMVs have diameters that vary from 20 to 250 nanometers, which allows it to carry great amount of substance. Estimated Number of Cas9 Encapsulation by OMVs
PICGIF LITE SOFTWARE
The display is created using the free online software Jmol to vistualize the 3D structure of CjCas9, with the help of PicGIF Lite to transform images into gif. Further explanation about the functions of these region can be found at Yomada 2017. The cyan RuvC domain, the blue PLL domain, the yellow WED domain and the incarnadine PI domain form the NUC(nuclease) domain. The white REC1 domain and the grey REC2 domain form the REC (crRNA recognition) domain. We have annotated the protein by color according to their specific functions. To provide a better view of the structure and functional domain of CjCas9, we provide a 360-degree rotating graph of the protein with its sgRNA and target DNA.įigure.1 Structural image of CjCas9 in complex with sgRNA(red) and target DNA(purple) sequence (with PAM AGAAACC). We provide models aiming to provide better understanding of our project and result analysis 1.
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